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International
Microarray Workshop: May 2005
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PLANT LONG-OLIGONUCLEOTIDE
MICROARRAY WORKSHOP
May 8-13, 2005
University of Arizona
Tucson, Arizona
This workshop comprised a
combination of lectures and hand-on laboratory sessions. The
participants primarily employed Arabidopsis and maize whole genome
70-mer oligonucleotide microarrays in their laboratory work (for
details of these arrays see http://www.ag.arizona.edu/microarray and
http://www.maizearray.org/). The workshop was aimed at the goal
of
obtaining optimal results using oligonucleotide-based
microarrays. It was offered concurrently with a workshop
focusing on animal long oligonucleotide microarrays.
The workshop was divided into two parts: Part I covered
wet-lab aspects of microarray target production, microarray
hybridization, and scanning. It started with registration on
Sunday, May 8, from 2-4PM, with the first formal activities at
5PM, and ended on Wednesday evening. Part II covered data
extraction, statistical analysis, and experimental design, and occupied
Thursday and Friday. Part I activities (plant and animal systems)
were offered
in separate laboratories, whereas Part II activities were offered
jointly.
Specific topics covered included:
- Experimental
design.
- Probe
preparation and microarray printing.
- Microarray
rehydration and probe immobilization.
- Target
preparation, including RNA extraction, direct and indirect labeling,
and amplification techniques.
- Microarray
hybridization.
- Array
scanning and data extraction.
- Data
analysis and archiving.
For further details of future workshops please contact David Galbraith.
Microarray
Workshop photographs
Microarray
Workshop data
D.W.
Galbraith
University of Arizona
Department of Plant Sciences
303 Forbes Building
Tucson Arizona 85721
Tel: (520) 621-9153; Fax (520) 621-7186
Page
last updated June 10, 2005
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