Growing Arabidopsis pollen (different methods)

* Pollen growth protocol (.doc)

Note: A key change to the protocol, (pH of the medium should be 7 for good pollen tube growth) has been incorporated. If you were having trouble previously, try the updated protocol.

Additional papers on Arabiodopsis pollen growth:

1. Boavida and McCormick (2007), Plant Journal, 52: 570–582 (Download pdf)

2. Firas Bou Daher, Youssef Chebli and Anja Geitmann (2009), Plant Cell Rep 28 (3): 347-357 (Download pdf)

Protocols to observe pollen tube growth within pistils

* Aniline blue staining protocol (.pdf)

* Congo red staining protocol (.pdf)

Protocol to prepare pollen tubes for observation by optical and electron microscopy

Chebli et al (2008), Microscopical Society of Canada bulletin 36(3) : 15-19 (Download pdf)

Additional protocols can be found here:

* Daphne Preuss lab website